Planning and carrying out time-resolved experiments

The Action co-organised a number of general and specific biophysical methods training schools, meetings and workshops. Details can be found on the Events and Past Events pages.  These often included pedagogic presentations that addressed time-resolved experiments. In addition, WG1 and WG3 organised several dedicated training schools and workshops, as well as publishing peer-reviewed papers and reviews that provided further details of experiment design and best practices in data analysis.

WG1

During the Action WG1 carried out a number of activities aimed at training and supporting the life science community in carrying out time-resolved structural experiments.

In addition, several papers providing guidance on time-resolved structural experiments were published by WG1:

  • Monteiro et al reported details of a microfluidic flow-focussed device for low sample consumption time-resolved scattering and diffraction experiments at synchrotrons.
  • Monteiro et al reported a light-triggered time-resolved SAXS paper in IUCrJ (2018) describing how to carry out a photocage compound initiated SAXS experiment to follow protein dimerisation.
  • Levantino et al published a review article in Current Opinion in Structural Biology (2015) which contained details of how to plan and carry out time-resolved SAXS and crystallographic experiments.

Finally a number of STSMs to Hamburg and ESRF from other Action participants were used to train students/postdocs in how to do time-resolved experiments, as well as develop specific protocols for each person’s system of interest.

WG3

WG3 was also active during the Action, training Action members in the use of state-of-the-art spectroscopic approaches to study biomacromoecular dynamics.

Several papers providing guidance and case studies for the use of spectroscopic tools to analyse protein dynamics were also published by WG3

  • Mullen et al published a review discussing the use of EPR to study protein dynamics.
  • Hellenkamp et al published a detailed benchamarking study to support establishing guidelines and best-practice for both data collection and analysis of FRET data for the study of protein structure and dynamics.

WG3 were also active in STSMs across Europe, providing tailored training and support for the projects of individual Action members that aimed to use or learn to use spectroscopic tools.